Label-free microcantilever-based immunosensors for highly sensitive determination of avian influenza virus H9
Identifieur interne : 000745 ( new/Analysis ); précédent : 000744; suivant : 000746Label-free microcantilever-based immunosensors for highly sensitive determination of avian influenza virus H9
Auteurs : Dan Xu [République populaire de Chine] ; Lin Liu [République populaire de Chine] ; Jun Guan [République populaire de Chine] ; Jianyun Xu [République populaire de Chine] ; Ting Wang [République populaire de Chine] ; Aijian Qin [République populaire de Chine] ; Xiaoya Hu [République populaire de Chine] ; Chengyin Wang [République populaire de Chine]Source :
- Mikrochimica Acta [ 0026-3672 ] ; 2013.
Abstract
We report on label-free immunosensors for the highly sensitive detection of avian influenza virus. The method makes use of the microcantilevers of an atomic force microscope onto which monoclonal antibodies against avian influenza virus were covalently immobilized. The factors influencing the performance of the resulting immunosensors were optimized by measuring the deflections of the cantilever via optical reflection, and this resulted in low detection limits and a wide analytical range. The differential deflection signals revealed specific antigen binding and their intensity is proportional to the logarithm of the concentrations of the virus in solution. Under optimal conditions, the immunosensors exhibit a linear response in the 7.6 ng mL−1 to 76 μg mL−1 concentration range of avian influenza virus, and the detection limit is 1.9 ng mL−1.
Label-free immunosensors based on microcantilevers of an atomic force microscope was fabricated by covalently immobilizing monoclonal antibodies to avian influenza virus onto the microcantilever. The performance and factors influencing the performance of the resulting immunosensors were investigated in detail by measuring the cantilever deflections using the optical reflection technique.
The online version of this article (doi:10.1007/s00604-013-1129-x) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00604-013-1129-x
PubMed: NONE
PubMed Central: 7087674
Affiliations:
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Yangzhou, 225002 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Chengyin" sort="Wang, Chengyin" uniqKey="Wang C" first="Chengyin" last="Wang">Chengyin Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Chemistry and Chemical Engineering,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, 225002 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yangzhou</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou, 225002 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author></analytic><series><title level="j">Mikrochimica Acta</title><idno type="ISSN">0026-3672</idno><idno type="eISSN">1436-5073</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We report on label-free immunosensors for the highly sensitive detection of avian influenza virus. The method makes use of the microcantilevers of an atomic force microscope onto which monoclonal antibodies against avian influenza virus were covalently immobilized. The factors influencing the performance of the resulting immunosensors were optimized by measuring the deflections of the cantilever via optical reflection, and this resulted in low detection limits and a wide analytical range. The differential deflection signals revealed specific antigen binding and their intensity is proportional to the logarithm of the concentrations of the virus in solution. Under optimal conditions, the immunosensors exhibit a linear response in the 7.6 ng mL<sup>−1</sup> to 76 μg mL<sup>−1</sup> concentration range of avian influenza virus, and the detection limit is 1.9 ng mL<sup>−1</sup>.</p><p><fig position="anchor" id="Figa"><label>Figure</label><caption><p>Label-free immunosensors based on microcantilevers of an atomic force microscope was fabricated by covalently immobilizing monoclonal antibodies to avian influenza virus onto the microcantilever. The performance and factors influencing the performance of the resulting immunosensors were investigated in detail by measuring the cantilever deflections using the optical reflection technique.</p></caption><graphic position="anchor" xlink:href="604_2013_1129_Figa_HTML" id="MO1"></graphic></fig></p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s00604-013-1129-x) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Velusamy, V" uniqKey="Velusamy V">V Velusamy</name></author><author><name sortKey="Arshak, K" uniqKey="Arshak K">K Arshak</name></author><author><name sortKey="Korostynska, O" uniqKey="Korostynska O">O Korostynska</name></author><author><name sortKey="Oliwa, K" uniqKey="Oliwa K">K Oliwa</name></author><author><name sortKey="Adley, C" uniqKey="Adley C">C 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Sheng</name></author><author><name sortKey="Chen, K" uniqKey="Chen K">K Chen</name></author><author><name sortKey="Guo, Xb" uniqKey="Guo X">XB Guo</name></author><author><name sortKey="Jin, Ml" uniqKey="Jin M">ML Jin</name></author><author><name sortKey="Han, Hy" uniqKey="Han H">HY Han</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, D" uniqKey="Guo D">D Guo</name></author><author><name sortKey="Zhuo, M" uniqKey="Zhuo M">M Zhuo</name></author><author><name sortKey="Zhang, Xa" uniqKey="Zhang X">XA Zhang</name></author><author><name sortKey="Xu, C" uniqKey="Xu C">C Xu</name></author><author><name sortKey="Jiang, J" uniqKey="Jiang J">J Jiang</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F Gao</name></author><author><name sortKey="Wan, Q" uniqKey="Wan Q">Q Wan</name></author><author><name sortKey="Lia, Qh" uniqKey="Lia Q">QH Lia</name></author><author><name sortKey="Wang, Th" uniqKey="Wang T">TH Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Rh" uniqKey="Wang R">RH Wang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Lassiter, K" uniqKey="Lassiter K">K Lassiter</name></author><author><name sortKey="Li, Yb" uniqKey="Li Y">YB Li</name></author><author><name sortKey="Hargis, B" uniqKey="Hargis B">B Hargis</name></author><author><name sortKey="Tung, S" uniqKey="Tung S">S Tung</name></author><author><name sortKey="Berghman, L" uniqKey="Berghman L">L Berghman</name></author><author><name sortKey="Bottje, W" uniqKey="Bottje W">W Bottje</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Rh" uniqKey="Wang R">RH Wang</name></author><author><name sortKey="Zhao, Jj" uniqKey="Zhao J">JJ Zhao</name></author><author><name sortKey="Jiang, Ts" uniqKey="Jiang T">TS Jiang</name></author><author><name sortKey="Kwon, Ym" uniqKey="Kwon Y">YM Kwon</name></author><author><name sortKey="Lu, Hg" uniqKey="Lu H">HG Lu</name></author><author><name sortKey="Jiao, Pr" uniqKey="Jiao P">PR Jiao</name></author><author><name sortKey="Liao, M" uniqKey="Liao M">M Liao</name></author><author><name sortKey="Li, Yb" uniqKey="Li Y">YB Li</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Dan" sort="Xu, Dan" uniqKey="Xu D" first="Dan" last="Xu">Dan Xu</name></noRegion><name sortKey="Guan, Jun" sort="Guan, Jun" uniqKey="Guan J" first="Jun" last="Guan">Jun Guan</name><name sortKey="Hu, Xiaoya" sort="Hu, Xiaoya" uniqKey="Hu X" first="Xiaoya" last="Hu">Xiaoya Hu</name><name sortKey="Hu, Xiaoya" sort="Hu, Xiaoya" uniqKey="Hu X" first="Xiaoya" last="Hu">Xiaoya Hu</name><name sortKey="Liu, Lin" sort="Liu, Lin" uniqKey="Liu L" first="Lin" last="Liu">Lin Liu</name><name sortKey="Qin, Aijian" sort="Qin, Aijian" uniqKey="Qin A" first="Aijian" last="Qin">Aijian Qin</name><name sortKey="Wang, Chengyin" sort="Wang, Chengyin" uniqKey="Wang C" first="Chengyin" last="Wang">Chengyin Wang</name><name sortKey="Wang, Chengyin" sort="Wang, Chengyin" uniqKey="Wang C" first="Chengyin" last="Wang">Chengyin Wang</name><name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name><name sortKey="Xu, Jianyun" sort="Xu, Jianyun" uniqKey="Xu J" first="Jianyun" last="Xu">Jianyun Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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